This work demonstrates that DLS is an effective technique of studying protein interactions. By means of the concentration dependence of BSA diffusion coefficients, one can obtain the parameters of protein interactions and can find that protein bears a net effective charge of −9.0 e and has a Hamaker constant of 2.8 k B T. With the increase of ionic strength, Debye screening decreases, protein interaction changes from repulsion to attraction, and protein begins to aggregate. The concentration dependence of BSA diffusion coefficients is interpreted in the context of a two-body potential of mean force, which includes repulsive hard-sphere and Coulombic interactions and attractive dispersion. The result shows that the diffusion coefficient of protein grows up with increasing protein concentration, and when the ionic strength turns up gradually the diffusion coefficient decreases with protein concentration’s increasing. This dependency is expressed by the interaction parameter, which can be assessed experimentally by light scattering measurements and have been determined for the diffusion coefficient of BSA under different salt concentration conditions in the present work. Please follow the link to our light scattering theory page to learn more about dynamic and static light scattering theory.The concentration dependence of the diffusion coefficient of particles suspended in solution depends primarily on the occupied volume fraction and on repulsive and attractive forces. Owing to the technique’s high sensitivity towards large species, even small impurities caused by aggregation can be detected Owing to its large measurement range (0.5 nm – 1 µm) DLS closes the gap between the range of small soluble aggregates that are detected via size exclusion chromatography (SEC) and particles analyzed by methods such as light obscuration (LO) or micro flow imaging (MFI). ZentriForce Pharma offers DLS services under BSL 1 and 2.ĭynamic light scattering is known as a fast and sensitive screening method for aggregate / particle detection and therefore widely used within biopharmaceutical development. We routinely conduct biosimilarity and gene therapy studies and are furthermore experienced in the development of dynamic light scattering (DLS) methods for a large variety of samples. The nano-dispersed system is placed into the. A few examples of suitable systems could include aggregated proteins, pigment components, micelles or emulsion droplets suspended in a continuous phase. Zentriforce Pharma is a contract research organization that specializes in analytical services with a focus on hydrodynamic methods. Dynamic Light Scattering requires particles approximately less than a micron in size to be homogenously suspended in a fluid (aqueous or organic).
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